The in situ pyrolytic methylation method may effectively prevent the carboxylic functional groups from cracking in the process of pyrolytic methylation reaction and can be used to investigate the chemical composition of oxygen containing compounds in the petroleum and organic matter of source rocks. 这表明,采用该方法可以在热解过程中有效地保留脂肪酸中的羧基,并能在分子级水平上研究非烃组分中含氧化合物的组成、结构。